Key features and details
VEGF (Clone# SP07-01)
VEGF Recombinant Rabbit Monoclonal Antibody [SP07-01]
Tested Application : WB，IHC-P，IF-Cell，IF-Tissue，IP，FC
Species Reactivity : Human，Mouse，Rat
Catalog# BA0033
Product Name：Anti-VEGF Antibody (Clone# SP07-01)
Gene Name：VEGF
Source：Rabbit
Clonality：Monoclonal
Isotype：IgG
Species Reactivity：Human, Mouse, Rat
Tested Application：WB, IF-Cell, IF-Tissue ,IHC-P, IP,FC
Contents：1ug/ul in 1×TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Immunogen：Synthetic peptide within Human VEGF aa 183-232 / 232.
Concentration：1ug/ul
Purification：Protein A affinity purified
Observed MW：Predicted band size: 27 kDa
Document
Recommended Dilution Ratios
|
Application |
Dilution Ratios |
|
WB |
1:500-1:2,000 |
|
IF-Cell |
1:50-1:200 |
|
IHC-P |
1:200-1:1,000 |
|
IF-Tissue |
1:50-1:200 |
|
FC |
1:1,000 |
|
IP |
Use at an assay dependent concentration |
Storage
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
| Product size | 1 ml, 100 µl, 50 µl |
|---|
The onset of angiogenesis is believed to be an early event in tumorigenesis and may facilitate tumor progression and metastasis. Several growth factors with angiogenic activity have been described. These include fibroblast growth factors (FGFs), platelet derived growth factor (PDGF) and vascular endothelial growth factor (VEGF). VEGF is a dimeric glycoprotein with structural homology to PDGF. Several variants of VEGF have been described that arise by alternative mRNA splicing. It has been speculated that VEGF may function as a tumor angiogenesis factor in vivo because the expression pattern of VEGF is consistent with a role in embryonic angiogenesis. VEGF mRNA is formed in some primary tumors, VEGF is produced by tumor cell lines in vitro and VEGF mitogenic activity appears to be restricted to endothelial cells. A member of the PDGF receptor family, Flt, has been identified as a high-affinity receptor for VEGF.
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