Category Archives: Antibody knowledge

Flow Cytometry PrincipleFlow cytometry has a multitude of applications in science, but the basic principle of operation is fairly simple: Cells (or other particles) pass in single file in front of a laser which allows them to be detected, counted, and sorted. These particles are fluorescently labelled, and the laser excites the fluorescent tags which […]

IHC PrincipleWestern blotting, ELISAs, and IHC are all immunoassays, and so all share in common the same basic principle of operation: primary antibodies bind to the target protein of interest, then labelled secondary antibodies bind the primary antibody, allowing for the precise detection of protein.IHC is the application of the immunoassay principle to tissue staining. […]

Western Blotting PrincipleWestern blotting, ELISAs, and IHC are all immunoassays, and so all share in common the same basic principle of operation: primary antibodies bind to the target protein of interest, then labelled secondary antibodies bind the primary antibody, allowing for the precise detection of protein.Western blotting differs from other immunoassays by employing gel electrophoresis […]

ELISA PrincipleWestern blotting, ELISAs, and IHC are all immunoassays, and so all share in common the same basic principle of operation: primary antibodies bind to the target protein of interest, then labelled secondary antibodies bind the primary antibody, allowing for the precise detection of protein. ELISAs are much more precise and sensitive than other forms […]