Category Archives: Antibody knowledge

IHC PrincipleWestern blotting, ELISAs, and IHC are all immunoassays, and so all share in common the same basic principle of operation: primary antibodies bind to the target protein of interest, then labelled secondary antibodies bind the primary antibody, allowing for the precise detection of protein.IHC is the application of the immunoassay principle to tissue staining. […]

Western Blotting PrincipleWestern blotting, ELISAs, and IHC are all immunoassays, and so all share in common the same basic principle of operation: primary antibodies bind to the target protein of interest, then labelled secondary antibodies bind the primary antibody, allowing for the precise detection of protein.Western blotting differs from other immunoassays by employing gel electrophoresis […]

ELISA PrincipleWestern blotting, ELISAs, and IHC are all immunoassays, and so all share in common the same basic principle of operation: primary antibodies bind to the target protein of interest, then labelled secondary antibodies bind the primary antibody, allowing for the precise detection of protein. ELISAs are much more precise and sensitive than other forms […]