Key features and details
CD34 (Clone #SI16-01)
CD34 Recombinant Rabbit Monoclonal Antibody [SI16-01]
Tested Application :WB, IF-Cell, IF-Tissue, IHC-P, IP, mIHC, FC
Species Reactivity: Human, Mouse, Rat
Catalog# BA0002
Product Name: Anti-CD34 Antibody (Clone# SI16-01)
Gene Name: CD34
Source: Rabbit
Clonality: Monoclonal
Isotype: IgG
Species Reactivity: human, mouse, rat
Tested Application: WB, IF-Cell, IF-Tissue, IHC-P, IP, mIHC, FC
Contents: 1ug/ul in 1×TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Immunogen: Synthetic peptide within Human CD34
Concentration: 1ug/ul
Purification: Protein A affinity purified
Observed MW: Predicted band size: 41 kDa
Document
Recommended Dilution Ratios
|
Application |
Dilution Ratios |
|
WB |
1:2,000 |
|
IF-Tissue |
1:500-1:1,000 |
|
IF-Cell |
1:100 |
|
IHC-P |
1:400–1:10,000 |
|
FC |
1:1,000 |
|
IP |
1-2µg /sample |
|
mIHC |
1:1,000-1:2,000 |
Storage
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
| Product size | 1 ml, 100 µl, 50 µl |
|---|
CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD.CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30% of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12—-qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis.
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